{"id":3947,"date":"2026-10-14T17:23:24","date_gmt":"2026-10-14T16:23:24","guid":{"rendered":"https:\/\/wpcalc.com\/tr\/kimya\/kromatografi-tutma-faktoru-hesaplayici\/"},"modified":"2026-10-14T17:23:24","modified_gmt":"2026-10-14T16:23:24","slug":"kromatografi-tutma-faktoru-hesaplayici","status":"publish","type":"post","link":"https:\/\/wpcalc.com\/tr\/kimya\/kromatografi-tutma-faktoru-hesaplayici\/","title":{"rendered":"Kromatografi Tutma Fakt\u00f6r\u00fc Hesaplama"},"content":{"rendered":"<section class=\"not-prose calculator-box\" aria-labelledby=\"calculator-title\"><div class=\"flex items-baseline sm:items-center justify-between gap-2 sm:gap-3\"><div class=\"flex flex-col sm:flex-row sm:items-center gap-2\"><span class=\"icon icon-calculator text-primary-700! text-base! dark:text-primary-300!\" aria-hidden=\"true\"><\/span><h2 id=\"calculator-title\" class=\"text-lg font-display font-bold\">Retansiyon s\u00fcrelerinden retansiyon fakt\u00f6r\u00fc k\u2032 hesaplama<\/h2><\/div><div class=\"calcdash-favorite group\">\n  <button type=\"button\" class=\"favorite\" id=\"favorite\" data-favorite-id=\"3947\" data-favorite-title=\"Kromatografi Tutma Fakt\u00f6r\u00fc\" data-favorite-url=\"https:\/\/wpcalc.com\/tr\/kimya\/kromatografi-tutma-faktoru-hesaplayici\/\" data-favorite-excerpt=\"Analitin tutulma s\u00fcresi ve kolonun \u00f6l\u00fc zaman\u0131ndan kromatografik tutma fakt\u00f6r\u00fc k\u2032 de\u011ferini hesaplay\u0131n. Her iki s\u00fcreyi diledi\u011finiz birimle girin; k\u2032, d\u00fczeltilmi\u015f tutulma s\u00fcresi ve yorumu an\u0131nda kar\u015f\u0131n\u0131za gelsin.\" aria-label=\"Favorilere ekle\" data-favorite-icon=\"icon icon-spectrum\">\n    <span class=\"icon icon-shape-star-empty\" aria-hidden=\"true\"><\/span>\n  <\/button>\n  <div class=\"calcdash-favorite__tip\" aria-hidden=\"true\">\n   <span class=\"favorite-tooltip\" id=\"favorite-tooltip\"><\/span>\n  <\/div>\n  <span class=\"sr-only\" role=\"status\" id=\"favorite-status\"><\/span>\n<\/div><\/div><form action=\"https:\/\/wpcalc.com\/tr\/kimya\/kromatografi-tutma-faktoru-hesaplayici\/\" method=\"POST\" class=\"calculator\" id=\"calculator-3947\" data-post=\"3947\"><fieldset class=\"fieldset-input is-2\"><legend class=\"sr-only\">Giri\u015f alanlar\u0131<\/legend><div class=\"field has-term\" id=\"input-1\"><label for=\"field-1\">Retansiyon s\u00fcresi (tR)<\/label><div class=\"term\">tR<\/div>  <div class=\"absolute bottom-0 left-16 top-8 w-2 h-0.5 bg-blue-500\"><\/div><div class=\"field-group\"><input type=\"text\" name=\"retansiyon_sresi_tr\" id=\"field-1\" aria-describedby=\"calc-info-1\" data-number inputmode=\"decimal\" autocomplete=\"off\" spellcheck=\"false\" placeholder=\"e.g. 8.5\" min=\"0\" value=\"8.5\"\/><div class=\"grid grid-cols-1\"><select id=\"field-2\" name=\"birim\" aria-label=\"Birim\"><option value=\"min\" selected>min<\/option><option value=\"s\">s<\/option><option value=\"h\">h<\/option><\/select><svg class=\"pointer-events-none col-start-1 row-start-1 mr-2 size-5 self-center justify-self-end text-gray-500 sm:size-4\" viewBox=\"0 0 16 16\" fill=\"currentColor\" aria-hidden=\"true\" data-slot=\"icon\">\n              <path fill-rule=\"evenodd\" d=\"M4.22 6.22a.75.75 0 0 1 1.06 0L8 8.94l2.72-2.72a.75.75 0 1 1 1.06 1.06l-3.25 3.25a.75.75 0 0 1-1.06 0L4.22 7.28a.75.75 0 0 1 0-1.06Z\" clip-rule=\"evenodd\" \/>\n            <\/svg><\/div><\/div><small id=\"calc-info-1\">Enjeksiyondan analitin pik maksimumuna kadar ge\u00e7en s\u00fcre.<\/small><\/div><div class=\"field has-term\" id=\"input-2\"><label for=\"field-3\">\u00d6l\u00fc s\u00fcre (t0)<\/label><div class=\"term\">t0<\/div>  <div class=\"absolute bottom-0 left-16 top-8 w-2 h-0.5 bg-blue-500\"><\/div><div class=\"field-group\"><input type=\"text\" name=\"l_sre_t0\" id=\"field-3\" aria-describedby=\"calc-info-3\" data-number inputmode=\"decimal\" autocomplete=\"off\" spellcheck=\"false\" placeholder=\"e.g. 1.5\" min=\"0\" value=\"1.5\"\/><div class=\"grid grid-cols-1\"><select id=\"field-4\" name=\"birim\" aria-label=\"Birim\"><option value=\"min\" selected>min<\/option><option value=\"s\">s<\/option><option value=\"h\">h<\/option><\/select><svg class=\"pointer-events-none col-start-1 row-start-1 mr-2 size-5 self-center justify-self-end text-gray-500 sm:size-4\" viewBox=\"0 0 16 16\" fill=\"currentColor\" aria-hidden=\"true\" data-slot=\"icon\">\n              <path fill-rule=\"evenodd\" d=\"M4.22 6.22a.75.75 0 0 1 1.06 0L8 8.94l2.72-2.72a.75.75 0 1 1 1.06 1.06l-3.25 3.25a.75.75 0 0 1-1.06 0L4.22 7.28a.75.75 0 0 1 0-1.06Z\" clip-rule=\"evenodd\" \/>\n            <\/svg><\/div><\/div><small id=\"calc-info-3\">Tutulmayan bir bile\u015fi\u011fin retansiyon s\u00fcresi (bo\u015fluk s\u00fcresi); \u00f6rne\u011fin ters faz LC&#039;de urasil veya GC&#039;de metan ile \u00f6l\u00e7\u00fcl\u00fcr.<\/small><\/div><\/fieldset><div class=\"buttons\"><button type=\"submit\" data-text=\"Yeniden hesapla\" id=\"calculate-button\" data-post=\"3947\">Hesapla<\/button><button type=\"reset\">S\u0131f\u0131rla<\/button><\/div><div class=\"field is-checkbox hidden!\" id=\"field-auto-calc\"><input type=\"checkbox\" id=\"auto-calc\"><label for=\"auto-calc\">Otomatik hesapla<\/label><small>Etkinle\u015ftirilirse, herhangi bir de\u011feri de\u011fi\u015ftirdi\u011finizde sonu\u00e7 otomatik olarak g\u00fcncellenir.<\/small><\/div><div class=\"fieldset-result is-hidden\" aria-labelledby=\"result-title\" aria-live=\"polite\" role=\"region\"> <h3 class=\"result-title bg-gradient-to-r from-primary-50 to-gray-50 dark:from-primary-900 dark:to-gray-800\" id=\"result-title\"><span class=\"icon icon-s-pulse\" aria-hidden=\"true\"><\/span> Retansiyon fakt\u00f6r\u00fc sonu\u00e7lar\u0131<\/h3><div class=\"result-box\"><div class=\"field-result\" id=\"output-1\"><span class=\"field-title\"><span>Retansiyon fakt\u00f6r\u00fc<\/span><span class=\"result-term\">k\u2032<\/span><\/span><span class=\"field-value\" id=\"result-1\"><\/span><button type=\"button\" class=\"copy-result\" data-tooltip=\"Sonucu kopyala\" aria-label=\"Sonucu kopyala\"><span class=\"copy-icon icon icon-document-copy\" aria-hidden=\"true\"><\/span><\/button><\/div><div class=\"field-result\" id=\"output-2\"><span class=\"field-title\"><span>D\u00fczeltilmi\u015f retansiyon s\u00fcresi<\/span><span class=\"result-term\">tR\u2032<\/span><\/span><span class=\"field-value\" id=\"result-2\"><\/span><button type=\"button\" class=\"copy-result\" data-tooltip=\"Sonucu kopyala\" aria-label=\"Sonucu kopyala\"><span class=\"copy-icon icon icon-document-copy\" aria-hidden=\"true\"><\/span><\/button><\/div><div class=\"field-result is-column\" id=\"output-3\"><span class=\"field-title\"><span>Hesaplama ayr\u0131nt\u0131lar\u0131<\/span><\/span><span class=\"field-value\" id=\"result-3\"><\/span><button type=\"button\" class=\"copy-result\" data-tooltip=\"Sonucu kopyala\" aria-label=\"Sonucu kopyala\"><span class=\"copy-icon icon icon-document-copy\" aria-hidden=\"true\"><\/span><\/button><\/div><\/div><div class=\"result-actions\"><button type=\"button\" class=\"copy-link\"><svg viewBox=\"0 0 20 20\" fill=\"currentColor\" aria-hidden=\"true\"><path d=\"M12.232 4.232a2.5 2.5 0 0 1 3.536 3.536l-1.225 1.224a.75.75 0 0 0 1.061 1.06l1.224-1.224a4 4 0 0 0-5.656-5.656l-3 3a4 4 0 0 0 .225 5.865.75.75 0 0 0 .977-1.138 2.5 2.5 0 0 1-.142-3.667l3-3Z\"\/><path d=\"M11.603 7.963a.75.75 0 0 0-.977 1.138 2.5 2.5 0 0 1 .142 3.667l-3 3a2.5 2.5 0 0 1-3.536-3.536l1.225-1.224a.75.75 0 0 0-1.061-1.06l-1.224 1.224a4 4 0 1 0 5.656 5.656l3-3a4 4 0 0 0-.225-5.865Z\"\/><\/svg><span class=\"copy-link__text\">Bu hesaplaman\u0131n ba\u011flant\u0131s\u0131n\u0131 kopyala<\/span><\/button><span class=\"sr-only\" role=\"status\" id=\"copy-link-status\"><\/span><\/div><\/div><a href=\"#respond\" class=\"hidden transition-opacity duration-300 opacity-0 w-50 text-sm justify-center items-center gap-2 px-4 py-2 rounded bg-gray-200 text-gray-700 hover:bg-gray-300\" id=\"leave-comment\"><span class=\"icon icon-comments\" aria-hidden=\"true\"><\/span>Yorum b\u0131rak<\/a><\/form><\/section><section id=\"calc-reactions\" class=\"not-prose hidden my-12 bg-gradient-to-r from-primary-50 to-gray-50 border border-indigo-100 rounded-xl px-6 py-4 shadow-sm\" data-post=\"3947\" aria-live=\"polite\" aria-labelledby=\"calc-reactions-title\"><p id=\"calc-reactions-title\" class=\"text-sm text-gray-500 text-center mb-2\">Bu sonu\u00e7 hakk\u0131nda ne d\u00fc\u015f\u00fcn\u00fcyorsunuz?<\/p><div role=\"group\" aria-labelledby=\"calc-reactions-title\" class=\"grid grid-cols-3 sm:flex sm:flex-row sm:justify-around gap-4 sm:items-center sm:flex-wrap\"><button type=\"button\" aria-pressed=\"false\" class=\"reaction-btn flex flex-col items-center gap-1 text-sm text-gray-700 hover:text-primary-600 cursor-pointer transition-transform hover:scale-105\" data-reaction=\"like\"><span class=\"reaction-count\" data-reaction-count=\"like\">0<\/span><span class=\"reaction text-3xl\" aria-hidden=\"true\">\ud83d\ude00 <\/span><span class=\"reaction-description font-medium\">Be\u011fendim <\/span><\/button><button type=\"button\" aria-pressed=\"false\" class=\"reaction-btn flex flex-col items-center gap-1 text-sm text-gray-700 hover:text-primary-600 cursor-pointer transition-transform hover:scale-105\" data-reaction=\"helpful\"><span class=\"reaction-count\" data-reaction-count=\"helpful\">0<\/span><span class=\"reaction text-3xl\" aria-hidden=\"true\">\ud83d\udca1 <\/span><span class=\"reaction-description font-medium\">Faydal\u0131 <\/span><\/button><button type=\"button\" aria-pressed=\"false\" class=\"reaction-btn flex flex-col items-center gap-1 text-sm text-gray-700 hover:text-primary-600 cursor-pointer transition-transform hover:scale-105\" data-reaction=\"confused\"><span class=\"reaction-count\" data-reaction-count=\"confused\">0<\/span><span class=\"reaction text-3xl\" aria-hidden=\"true\">\ud83d\ude15 <\/span><span class=\"reaction-description font-medium\">Kafam kar\u0131\u015ft\u0131 <\/span><\/button><button type=\"button\" aria-pressed=\"false\" class=\"reaction-btn flex flex-col items-center gap-1 text-sm text-gray-700 hover:text-primary-600 cursor-pointer transition-transform hover:scale-105\" data-reaction=\"disappointed\"><span class=\"reaction-count\" data-reaction-count=\"disappointed\">0<\/span><span class=\"reaction text-3xl\" aria-hidden=\"true\">\ud83d\ude1e <\/span><span class=\"reaction-description font-medium\">Hayal k\u0131r\u0131kl\u0131\u011f\u0131 <\/span><\/button><button type=\"button\" aria-pressed=\"false\" class=\"reaction-btn flex flex-col items-center gap-1 text-sm text-gray-700 hover:text-primary-600 cursor-pointer transition-transform hover:scale-105\" data-reaction=\"inaccurate\"><span class=\"reaction-count\" data-reaction-count=\"inaccurate\">0<\/span><span class=\"reaction text-3xl\" aria-hidden=\"true\">\u274c <\/span><span class=\"reaction-description font-medium\">Hatal\u0131 <\/span><\/button><\/div><div id=\"reaction-message\" class=\"hidden mt-8 rounded-md border border-primary-100 bg-white\/50 backdrop-blur-sm px-4 py-2 text-sm text-center shadow-sm\"><\/div><\/section><div class=\"calcdash-ad not-prose calcdash-ad--calc_result calcdash-ad--in-article\"><ins class=\"adsbygoogle\" style=\"display:block\" data-ad-client=\"ca-pub-1721569815777345\" data-ad-slot=\"6317458308\" data-ad-layout=\"in-article\" data-ad-format=\"fluid\"><\/ins><script>(adsbygoogle = window.adsbygoogle || []).push({});<\/script><\/div>\n<style id=\"wpcoderpro-inline-1\">.formula-box {\r\n\tborder-bottom: none !important;\r\n}<\/style><section class=\"formula-box\" aria-labelledby=\"formula-title\">\r\n\t<div id=\"formula-title\" class=\"not-prose font-display mb-4\"><span class=\"icon icon-formula\" aria-hidden=\"true\"><\/span><h2>Tutma Fakt\u00f6r\u00fc Form\u00fcl\u00fc<\/h2><\/div>\r\n\t<figure class=\"not-prose formula\">\r\n\t\t<figcaption class=\"formula-title\">Form\u00fcl<\/figcaption>\r\n\t\t<div class=\"text-base text-gray-800\" id=\"formula\">\\[ k' = \\frac{t_R - t_0}{t_0} = \\frac{t_R'}{t_0} \\]<\/div>\r\n\t<\/figure>\r\n\t<\/p>\n<p><strong>Burada:<\/strong><\/p>\n<ul>\n<li>\\(k'\\) \u2014 tutma fakt\u00f6r\u00fc (kapasite fakt\u00f6r\u00fc olarak da adland\u0131r\u0131l\u0131r, boyutsuzdur)<\/li>\n<li>\\(t_R\\) \u2014 analitin tutulma s\u00fcresi: enjeksiyondan pikin tepe noktas\u0131na kadar ge\u00e7en s\u00fcre<\/li>\n<li>\\(t_0\\) \u2014 \u00f6l\u00fc zaman (bo\u015f zaman): kolonda hi\u00e7 tutulmayan bir bile\u015fi\u011fin tutulma s\u00fcresi<\/li>\n<li>\\(t_R'\\) \u2014 d\u00fczeltilmi\u015f tutulma s\u00fcresi: \\(t_R' = t_R - t_0\\)<\/li>\n<\/ul>\n<p>Form\u00fcl, analitin durgun faz taraf\u0131ndan, kolondan hi\u00e7 al\u0131konmadan ge\u00e7en bir bile\u015fi\u011fe k\u0131yasla ne kadar daha uzun s\u00fcre geciktirildi\u011fini ifade eder. \u0130ki s\u00fcrenin oran\u0131 oldu\u011fu i\u00e7in \\(k'\\) boyutsuzdur ve ak\u0131\u015f h\u0131z\u0131ndan ba\u011f\u0131ms\u0131zd\u0131r.<\/p>\n<p>\r\n<\/section>\n<style id=\"wpcoderpro-inline-2\">.calculation-box {\r\n\tborder-bottom: none !important;\r\n}<\/style><section id=\"calculation\" class=\"calculation-box\" aria-labelledby=\"calculation-title\">\r\n\t<div id=\"calculation-title\" class=\"not-prose font-display\"><span class=\"icon icon-unordered-list\" aria-hidden=\"true\"><\/span><h2>Tutma Fakt\u00f6r\u00fc \u2013 \u00d6rnek Hesaplama<\/h2><\/div>\r\n\r\n<\/p>\n<p><strong>\u00d6rnek:<\/strong> bir analit pikinin tutulma s\u00fcresi 8,5 dk, tutulmayan i\u015faretleyici bile\u015fik ise (\u00f6rne\u011fin ters faz LC'de urasil) 1,5 dk'da \u00e7\u0131k\u0131yor.<\/p>\n<p>\\[ k' = \\frac{t_R - t_0}{t_0} = \\frac{8.5 - 1.5}{1.5} = \\frac{7.0}{1.5} \\approx 4.6667 \\]<\/p>\n<p><strong>Sonu\u00e7:<\/strong> tutma fakt\u00f6r\u00fc \\(k' \\approx 4.67\\), d\u00fczeltilmi\u015f tutulma s\u00fcresi ise \\(t_R' = 8.5 - 1.5 = 7.0\\) dk. Analit, yaln\u0131zca hareketli fazda ilerleseydi harcayaca\u011f\u0131 s\u00fcrenin yakla\u015f\u0131k 4,67 kat\u0131 kadar bir s\u00fcre boyunca durgun fazla etkile\u015fiyor \u2014 bu de\u011fer, izokratik HPLC i\u00e7in al\u0131\u015f\u0131lm\u0131\u015f 1\u201310 aral\u0131\u011f\u0131ndaki optimum band\u0131n\u0131n rahat\u00e7a i\u00e7indedir.<\/p>\n<p>\n\r\n<\/section>\n<h2>Hesaplay\u0131c\u0131 Nas\u0131l Kullan\u0131l\u0131r?<\/h2>\n<ol>\n<li>Analitinizin <strong>tutulma s\u00fcresini (tR)<\/strong> girin \u2014 enjeksiyondan pikin tepesine kadar ge\u00e7en s\u00fcre.<\/li>\n<li>Alan\u0131n yan\u0131ndaki a\u00e7\u0131l\u0131r listeden uygun birimi (dk, sn veya saat) se\u00e7in.<\/li>\n<li><strong>\u00d6l\u00fc zaman\u0131 (t0)<\/strong> girin; bu, tutulmayan bir bile\u015fi\u011fin tutulma s\u00fcresidir. Birimini de se\u00e7in. \u0130ki s\u00fcre farkl\u0131 birimlerde verilebilir; hesaplay\u0131c\u0131 bunlar\u0131 otomatik olarak d\u00f6n\u00fc\u015ft\u00fcr\u00fcr.<\/li>\n<li><strong>Tutma fakt\u00f6r\u00fcn\u00fc<\/strong> \\(k'\\), <strong>d\u00fczeltilmi\u015f tutulma s\u00fcresini<\/strong> \\(t_R'\\) (dakika cinsinden) ve yerine koyma ad\u0131mlar\u0131yla birlikte sonucun yorumunu g\u00f6steren <strong>hesaplama ayr\u0131nt\u0131lar\u0131n\u0131<\/strong> okuyun.<\/li>\n<\/ol>\n<h2>Tutma Fakt\u00f6r\u00fc Nedir?<\/h2>\n<p>Tutma fakt\u00f6r\u00fc \\(k'\\) (kimi zaman \\(k\\) olarak yaz\u0131l\u0131r, eskiden kapasite fakt\u00f6r\u00fc \\(k'\\) diye an\u0131l\u0131rd\u0131), bir analitin kromatografik kolon taraf\u0131ndan ne kadar g\u00fc\u00e7l\u00fc tutuldu\u011funu betimlemenin standart yoludur. Analitin durgun faz taraf\u0131ndan geciktirildi\u011fi s\u00fcreyi (d\u00fczeltilmi\u015f tutulma s\u00fcresi \\(t_R'\\)) yaln\u0131zca hareketli fazla birlikte yol alan herhangi bir maddenin harcad\u0131\u011f\u0131 s\u00fcreyle (\u00f6l\u00fc zaman \\(t_0\\)) kar\u015f\u0131la\u015ft\u0131r\u0131r.<\/p>\n<p>Ham tutulma s\u00fcreleri pratik g\u00f6r\u00fcnse de yan\u0131lt\u0131c\u0131d\u0131r: ak\u0131\u015f h\u0131z\u0131n\u0131, kolon uzunlu\u011funu veya cihaz\u0131 de\u011fi\u015ftirdi\u011finizde ayn\u0131 ayr\u0131\u015fma farkl\u0131 s\u00fcreler \u00fcretir. Tutma fakt\u00f6r\u00fc ise hem hareketli fazda ge\u00e7en s\u00fcreyi hem de ak\u0131\u015f h\u0131z\u0131n\u0131n etkisini ortadan kald\u0131r\u0131r; b\u00f6ylece \\(k'\\) de\u011ferleri y\u00f6ntemler aras\u0131nda kar\u015f\u0131la\u015ft\u0131r\u0131labilir ve literat\u00fcrde analit\u2013durgun faz\u2013hareketli faz sisteminin bir \u00f6zelli\u011fi olarak raporlanabilir. Farmakopelerin ve y\u00f6ntem validasyon k\u0131lavuzlar\u0131n\u0131n tutmay\u0131 dakika yerine \\(k'\\) cinsinden tan\u0131mlamas\u0131n\u0131n nedeni de budur.<\/p>\n<p>\u0130\u015fe yarar bir fiziksel yorumu da vard\u0131r: \\(k'\\), analitin durgun fazda ge\u00e7irdi\u011fi ortalama s\u00fcrenin (veya miktar\u0131n) hareketli fazdakine oran\u0131d\u0131r. Bir analit molek\u00fcl\u00fc hareketli fazda zaman\u0131n\u0131n \\(1\/(1+k')\\), durgun fazda ise \\(k'\/(1+k')\\) kadar\u0131n\u0131 ge\u00e7irir. Dolay\u0131s\u0131yla 4,67'lik bir \\(k'\\), analitin zaman\u0131n\u0131n yakla\u015f\u0131k %82'sini durgun faz taraf\u0131ndan tutularak ge\u00e7irdi\u011fi anlam\u0131na gelir.<\/p>\n<p>Tutma fakt\u00f6r\u00fc ayr\u0131ca kromatografinin di\u011fer temel b\u00fcy\u00fckl\u00fcklerine do\u011frudan girer: ay\u0131rma derinli\u011fi (\u00e7\u00f6z\u00fcn\u00fcrl\u00fck) denkleminde, se\u00e7icilik \\(\\alpha = k_2'\/k_1'\\) i\u00e7inde ve da\u011f\u0131l\u0131m katsay\u0131s\u0131yla ili\u015fkide \\(k' = K \\cdot V_s \/ V_m\\) bi\u00e7iminde yer al\u0131r. Ay\u0131rman\u0131n genel kalitesini de\u011ferlendiriyorsan\u0131z \\(k'\\) de\u011ferini pik geni\u015flikleriyle birlikte <a href=\"https:\/\/wpcalc.com\/tr\/kimya\/kromatografi-rezolusyon-hesaplayici\/\">Kromatografi \u00c7\u00f6z\u00fcn\u00fcrl\u00fck hesaplay\u0131c\u0131m\u0131zda<\/a> kullan\u0131n; d\u00fczlemsel kromatografide kar\u015f\u0131l\u0131k gelen b\u00fcy\u00fckl\u00fck Rf de\u011feridir ve onu <a href=\"https:\/\/wpcalc.com\/tr\/kimya\/itk-rf-hesaplama\/\">TLC Rf hesaplay\u0131c\u0131s\u0131yla<\/a> hesaplayabilirsiniz.<\/p>\n<h2>\u00d6l\u00fc Zaman\u0131n (t0) \u00d6l\u00e7\u00fcm\u00fc<\/h2>\n<p>\\(k'\\) do\u011frudan \\(t_0\\)'a ba\u011fl\u0131 oldu\u011fundan, yanl\u0131\u015f bir \u00f6l\u00fc zaman t\u00fcm sonu\u00e7lar\u0131 bozar. \u00d6l\u00fc zaman, hi\u00e7 tutulmayan bir bile\u015fik enjekte edilerek \u00f6l\u00e7\u00fcl\u00fcr:<\/p>\n<ul>\n<li><strong>Ters faz HPLC:<\/strong> klasik i\u015faretleyici urasildir; tiyo\u00fcre de yayg\u0131nd\u0131r.<\/li>\n<li><strong>Normal faz LC:<\/strong> pentan veya hekzan gibi \u00e7ok apolar bir \u00e7\u00f6z\u00fcc\u00fc \u00e7o\u011fu zaman bo\u015fluk i\u015faretleyicisi olarak kullan\u0131l\u0131r.<\/li>\n<li><strong>GC:<\/strong> sabit fazla hemen hemen hi\u00e7 etkile\u015fmeyen metan veya b\u00fctan kullan\u0131l\u0131r.<\/li>\n<\/ul>\n<p>Pratik bir alternatif, enjeksiyondan sonraki ilk taban \u00e7izgisi bozulmas\u0131n\u0131n zaman\u0131n\u0131 not etmektir (\"\u00e7\u00f6z\u00fcc\u00fc cephesi\" veya enjeksiyon piki); yine de ger\u00e7ek bir tutulmayan i\u015faretleyici daha g\u00fcvenilirdir.<\/p>\n<h2>k\u2032 De\u011ferini Yorumlamak ve Kullanmak<\/h2>\n<p>\u0130zokratik HPLC ay\u0131rmalar\u0131 i\u00e7in tipik k\u0131lavuz de\u011ferler:<\/p>\n<table>\n<thead>\n<tr>\n<th>k\u2032 aral\u0131\u011f\u0131<\/th>\n<th>Yorum<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>&lt; 1<\/td>\n<td>Zay\u0131f tutulur; pik, giri\u015fimlerin yo\u011funla\u015ft\u0131\u011f\u0131\u011f\u0131 ve \u00e7\u00f6z\u00fcc\u00fc cephesinden ayr\u0131\u015fman\u0131n zay\u0131f oldu\u011fu bo\u015fluk zaman\u0131na yak\u0131n \u00e7\u0131kar.<\/td>\n<\/tr>\n<tr>\n<td>1\u201310 (ideali 2\u20135)<\/td>\n<td>Al\u0131\u015f\u0131lm\u0131\u015f optimum \u00e7al\u0131\u015fma aral\u0131\u011f\u0131: a\u015f\u0131r\u0131 uzun analiz s\u00fcresine girmeden iyi \u00e7\u00f6z\u00fcn\u00fcrl\u00fck.<\/td>\n<\/tr>\n<tr>\n<td>&gt; 10<\/td>\n<td>G\u00fc\u00e7l\u00fc tutulur; uzun analizler, geni\u015f pikler ve d\u00fc\u015f\u00fck duyarl\u0131l\u0131k. Ters faz LC'de organik \u00e7\u00f6z\u00fcc\u00fc oran\u0131n\u0131 art\u0131r\u0131n ya da gradiyente ge\u00e7in.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>Ters faz LC'de \\(k'\\) de\u011ferini art\u0131rmak i\u00e7in daha zay\u0131f bir hareketli faz (daha az organik \u00e7\u00f6z\u00fcc\u00fc, daha fazla su), daha uzun veya daha tutucu bir kolon ya da daha d\u00fc\u015f\u00fck s\u0131cakl\u0131k kullan\u0131n. Azaltmak i\u00e7in tersini yap\u0131n. \\(k'\\) de\u011ferinin de\u011fi\u015fmesinin di\u011fer t\u00fcm piklerin tutulmas\u0131n\u0131 da kayd\u0131raca\u011f\u0131n\u0131 unutmay\u0131n; bu nedenle her ayarlama sonras\u0131nda \u00e7\u00f6z\u00fcn\u00fcrl\u00fc\u011f\u00fc yeniden kontrol edin.<\/p>\n<h2>Varsay\u0131mlar ve S\u0131n\u0131rlamalar<\/h2>\n<ul>\n<li>\\(t_R\\) ve \\(t_0\\) ayn\u0131 ko\u015fullarda \u00f6l\u00e7\u00fclmelidir \u2014 ayn\u0131 ak\u0131\u015f h\u0131z\u0131, s\u0131cakl\u0131k, hareketli faz ve kolon. Farkl\u0131 ko\u015fulara ait de\u011ferleri kar\u0131\u015ft\u0131rmak anlams\u0131z sonu\u00e7lar verir.<\/li>\n<li>\\(t_0\\) i\u00e7in kullan\u0131lan i\u015faretleyici ger\u00e7ekten tutulmayan bir bile\u015fik olmal\u0131d\u0131r. Hafif\u00e7e tutulan bir \"bo\u015fluk i\u015faretleyicisi\" \\(t_0\\)'\u0131 oldu\u011fundan b\u00fcy\u00fck, \\(k'\\) de\u011ferini oldu\u011fundan k\u00fc\u00e7\u00fck g\u00f6sterir.<\/li>\n<li>\\(t_R\\), \\(t_0\\)'dan b\u00fcy\u00fck olmal\u0131d\u0131r; bo\u015fluk i\u015faretleyicisinden \u00f6nce \u00e7\u0131kan bir pik bir anomaliye i\u015faret eder (ya da \"i\u015faretleyicinin\" ger\u00e7ekte tutulmad\u0131\u011f\u0131n\u0131n kan\u0131t\u0131d\u0131r).<\/li>\n<li>Basit s\u00fcre temelli form\u00fcl, hareketli faz bile\u015fiminin sabit oldu\u011funu varsayar (izokratik el\u00fcsyon). Gradiyan ko\u015fular\u0131nda \\(k'\\) ayr\u0131\u015fma boyunca de\u011fi\u015fir ve bu kavram\u0131n yerini gradiyan tutma fakt\u00f6r\u00fc ya da \\(k_e\\) al\u0131r.<\/li>\n<li>Tutma fakt\u00f6rleri s\u0131cakl\u0131\u011fa ba\u011fl\u0131d\u0131r; termodinamik \u00e7al\u0131\u015fmalarda \\(k'\\) de\u011ferinin s\u0131cakl\u0131k ba\u011f\u0131ml\u0131l\u0131\u011f\u0131 <a href=\"https:\/\/wpcalc.com\/tr\/kimya\/van-t-hoff-denklem-hesaplayici\/\">Van 't Hoff denklemi<\/a> ile \u00e7\u00f6z\u00fcmlenebilir.<\/li>\n<li>Bu b\u00fcy\u00fckl\u00fc\u011f\u00fcn temelindeki denge niceli\u011fi, iki faz aras\u0131ndaki <a href=\"https:\/\/wpcalc.com\/tr\/kimya\/bolusme-katsayisi-hesaplama\/\">b\u00f6l\u00fcnme katsay\u0131s\u0131d\u0131r<\/a>; tutmay\u0131 kimyasal yap\u0131yla ili\u015fkilendirirken i\u015finize yarayabilir.<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Analitin tutulma s\u00fcresi ve kolonun \u00f6l\u00fc zaman\u0131ndan kromatografik tutma fakt\u00f6r\u00fc k\u2032 de\u011ferini hesaplay\u0131n. Her iki s\u00fcreyi diledi\u011finiz birimle girin; k\u2032, d\u00fczeltilmi\u015f tutulma s\u00fcresi ve yorumu an\u0131nda kar\u015f\u0131n\u0131za gelsin.<\/p>\n","protected":false},"author":3168,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[3],"tags":[68],"class_list":["post-3947","post","type-post","status-publish","format-standard","hentry","category-kimya","tag-spektrofotometri"],"acf":[],"_links":{"self":[{"href":"https:\/\/wpcalc.com\/tr\/wp-json\/wp\/v2\/posts\/3947","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wpcalc.com\/tr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wpcalc.com\/tr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wpcalc.com\/tr\/wp-json\/wp\/v2\/users\/3168"}],"replies":[{"embeddable":true,"href":"https:\/\/wpcalc.com\/tr\/wp-json\/wp\/v2\/comments?post=3947"}],"version-history":[{"count":0,"href":"https:\/\/wpcalc.com\/tr\/wp-json\/wp\/v2\/posts\/3947\/revisions"}],"wp:attachment":[{"href":"https:\/\/wpcalc.com\/tr\/wp-json\/wp\/v2\/media?parent=3947"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wpcalc.com\/tr\/wp-json\/wp\/v2\/categories?post=3947"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wpcalc.com\/tr\/wp-json\/wp\/v2\/tags?post=3947"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}