{"id":3948,"date":"2026-10-14T17:27:57","date_gmt":"2026-10-14T16:27:57","guid":{"rendered":"https:\/\/wpcalc.com\/tr\/kimya\/kromatografi-secicilik-faktoru-hesaplama\/"},"modified":"2026-10-14T17:27:57","modified_gmt":"2026-10-14T16:27:57","slug":"kromatografi-secicilik-faktoru-hesaplama","status":"publish","type":"post","link":"https:\/\/wpcalc.com\/tr\/kimya\/kromatografi-secicilik-faktoru-hesaplama\/","title":{"rendered":"Kromatografi Se\u00e7icilik Fakt\u00f6r\u00fc Hesaplay\u0131c\u0131s\u0131"},"content":{"rendered":"<section class=\"not-prose calculator-box\" aria-labelledby=\"calculator-title\"><div class=\"flex items-baseline sm:items-center justify-between gap-2 sm:gap-3\"><div class=\"flex flex-col sm:flex-row sm:items-center gap-2\"><span class=\"icon icon-calculator text-primary-700! text-base! dark:text-primary-300!\" aria-hidden=\"true\"><\/span><h2 id=\"calculator-title\" class=\"text-lg font-display font-bold\">Kolonun iki bile\u015fi\u011fi ne kadar se\u00e7ici tuttu\u011funu kar\u015f\u0131la\u015ft\u0131r\u0131n<\/h2><\/div><div class=\"calcdash-favorite group\">\n  <button type=\"button\" class=\"favorite\" id=\"favorite\" data-favorite-id=\"3948\" data-favorite-title=\"Kromatografi Se\u00e7icilik Fakt\u00f6r\u00fc\" data-favorite-url=\"https:\/\/wpcalc.com\/tr\/kimya\/kromatografi-secicilik-faktoru-hesaplama\/\" data-favorite-excerpt=\"\u0130ki bile\u015fi\u011fin retansiyon s\u00fcrelerini ve kolonun \u00f6l\u00fc zaman\u0131n\u0131 girin; se\u00e7icilik fakt\u00f6r\u00fcn\u00fc (\u03b1) ve iki al\u0131konma fakt\u00f6r\u00fcn\u00fc hesaplay\u0131n, ayr\u0131ca kolonun bu \u00e7ifti ne kadar iyi ay\u0131rd\u0131\u011f\u0131n\u0131 g\u00f6r\u00fcn.\" aria-label=\"Favorilere ekle\" data-favorite-icon=\"icon icon-spectrum\">\n    <span class=\"icon icon-shape-star-empty\" aria-hidden=\"true\"><\/span>\n  <\/button>\n  <div class=\"calcdash-favorite__tip\" aria-hidden=\"true\">\n   <span class=\"favorite-tooltip\" id=\"favorite-tooltip\"><\/span>\n  <\/div>\n  <span class=\"sr-only\" role=\"status\" id=\"favorite-status\"><\/span>\n<\/div><\/div><form action=\"https:\/\/wpcalc.com\/tr\/kimya\/kromatografi-secicilik-faktoru-hesaplama\/\" method=\"POST\" class=\"calculator\" id=\"calculator-3948\" data-post=\"3948\"><fieldset class=\"fieldset-input is-2\"><legend class=\"sr-only\">Giri\u015f alanlar\u0131<\/legend><div class=\"field has-term\" id=\"input-1\"><label for=\"field-1\">1. bile\u015fi\u011fin al\u0131konma s\u00fcresi<\/label><div class=\"term\">tR1<\/div>  <div class=\"absolute bottom-0 left-16 top-8 w-2 h-0.5 bg-blue-500\"><\/div><div class=\"field-group\"><input type=\"text\" name=\"1_bileiin_alkonma_sresi\" id=\"field-1\" aria-describedby=\"calc-info-1\" data-number inputmode=\"decimal\" autocomplete=\"off\" spellcheck=\"false\" placeholder=\"e.g. 4.2\" min=\"0\" value=\"4.2\"\/><div class=\"grid grid-cols-1\"><select id=\"field-2\" name=\"birim\" aria-label=\"Birim\"><option value=\"min\" selected>min<\/option><option value=\"s\">s<\/option><\/select><svg class=\"pointer-events-none col-start-1 row-start-1 mr-2 size-5 self-center justify-self-end text-gray-500 sm:size-4\" viewBox=\"0 0 16 16\" fill=\"currentColor\" aria-hidden=\"true\" data-slot=\"icon\">\n              <path fill-rule=\"evenodd\" d=\"M4.22 6.22a.75.75 0 0 1 1.06 0L8 8.94l2.72-2.72a.75.75 0 1 1 1.06 1.06l-3.25 3.25a.75.75 0 0 1-1.06 0L4.22 7.28a.75.75 0 0 1 0-1.06Z\" clip-rule=\"evenodd\" \/>\n            <\/svg><\/div><\/div><small id=\"calc-info-1\">\u0130lk (daha erken elue edilen) pikin, enjeksiyon an\u0131ndan itibaren \u00f6l\u00e7\u00fclen al\u0131konma s\u00fcresi tR.<\/small><\/div><div class=\"field has-term\" id=\"input-2\"><label for=\"field-3\">2. bile\u015fi\u011fin al\u0131konma s\u00fcresi<\/label><div class=\"term\">tR2<\/div>  <div class=\"absolute bottom-0 left-16 top-8 w-2 h-0.5 bg-blue-500\"><\/div><div class=\"field-group\"><input type=\"text\" name=\"2_bileiin_alkonma_sresi\" id=\"field-3\" aria-describedby=\"calc-info-3\" data-number inputmode=\"decimal\" autocomplete=\"off\" spellcheck=\"false\" placeholder=\"e.g. 6.8\" min=\"0\" value=\"6.8\"\/><div class=\"grid grid-cols-1\"><select id=\"field-4\" name=\"birim\" aria-label=\"Birim\"><option value=\"min\" selected>min<\/option><option value=\"s\">s<\/option><\/select><svg class=\"pointer-events-none col-start-1 row-start-1 mr-2 size-5 self-center justify-self-end text-gray-500 sm:size-4\" viewBox=\"0 0 16 16\" fill=\"currentColor\" aria-hidden=\"true\" data-slot=\"icon\">\n              <path fill-rule=\"evenodd\" d=\"M4.22 6.22a.75.75 0 0 1 1.06 0L8 8.94l2.72-2.72a.75.75 0 1 1 1.06 1.06l-3.25 3.25a.75.75 0 0 1-1.06 0L4.22 7.28a.75.75 0 0 1 0-1.06Z\" clip-rule=\"evenodd\" \/>\n            <\/svg><\/div><\/div><small id=\"calc-info-3\">\u0130kinci (daha ge\u00e7 elue edilen) pikin, enjeksiyon an\u0131ndan itibaren \u00f6l\u00e7\u00fclen al\u0131konma s\u00fcresi tR.<\/small><\/div><div class=\"field has-term\" id=\"input-3\"><label for=\"field-5\">\u00d6l\u00fc s\u00fcre<\/label><div class=\"term\">t0<\/div>  <div class=\"absolute bottom-0 left-16 top-8 w-2 h-0.5 bg-blue-500\"><\/div><div class=\"field-group\"><input type=\"text\" name=\"l_sre\" id=\"field-5\" aria-describedby=\"calc-info-5\" data-number inputmode=\"decimal\" autocomplete=\"off\" spellcheck=\"false\" placeholder=\"e.g. 1.5\" min=\"0\" value=\"1.5\"\/><div class=\"grid grid-cols-1\"><select id=\"field-6\" name=\"birim\" aria-label=\"Birim\"><option value=\"min\" selected>min<\/option><option value=\"s\">s<\/option><\/select><svg class=\"pointer-events-none col-start-1 row-start-1 mr-2 size-5 self-center justify-self-end text-gray-500 sm:size-4\" viewBox=\"0 0 16 16\" fill=\"currentColor\" aria-hidden=\"true\" data-slot=\"icon\">\n              <path fill-rule=\"evenodd\" d=\"M4.22 6.22a.75.75 0 0 1 1.06 0L8 8.94l2.72-2.72a.75.75 0 1 1 1.06 1.06l-3.25 3.25a.75.75 0 0 1-1.06 0L4.22 7.28a.75.75 0 0 1 0-1.06Z\" clip-rule=\"evenodd\" \/>\n            <\/svg><\/div><\/div><small id=\"calc-info-5\">Kolonun \u00f6l\u00fc s\u00fcresi t0 (ge\u00e7me s\u00fcresi); yani \u00e7\u00f6z\u00fcc\u00fc cephesi gibi tutulmayan bir pikin al\u0131konma s\u00fcresi.<\/small><\/div><\/fieldset><div class=\"buttons\"><button type=\"submit\" data-text=\"Yeniden hesapla\" id=\"calculate-button\" data-post=\"3948\">Hesapla<\/button><button type=\"reset\">S\u0131f\u0131rla<\/button><\/div><div class=\"field is-checkbox hidden!\" id=\"field-auto-calc\"><input type=\"checkbox\" id=\"auto-calc\"><label for=\"auto-calc\">Otomatik hesapla<\/label><small>Etkinle\u015ftirilirse, herhangi bir de\u011feri de\u011fi\u015ftirdi\u011finizde sonu\u00e7 otomatik olarak g\u00fcncellenir.<\/small><\/div><div class=\"fieldset-result is-hidden\" aria-labelledby=\"result-title\" aria-live=\"polite\" role=\"region\"> <h3 class=\"result-title bg-gradient-to-r from-primary-50 to-gray-50 dark:from-primary-900 dark:to-gray-800\" id=\"result-title\"><span class=\"icon icon-s-pulse\" aria-hidden=\"true\"><\/span> Se\u00e7icilik Fakt\u00f6r\u00fc Sonu\u00e7lar\u0131<\/h3><div class=\"result-box\"><div class=\"field-result\" id=\"output-1\"><span class=\"field-title\"><span>Se\u00e7icilik fakt\u00f6r\u00fc<\/span><span class=\"result-term\">\u03b1<\/span><\/span><span class=\"field-value\" id=\"result-1\"><\/span><button type=\"button\" class=\"copy-result\" data-tooltip=\"Sonucu kopyala\" aria-label=\"Sonucu kopyala\"><span class=\"copy-icon icon icon-document-copy\" aria-hidden=\"true\"><\/span><\/button><\/div><div class=\"field-result\" id=\"output-2\"><span class=\"field-title\"><span>1. bile\u015fi\u011fin al\u0131konma fakt\u00f6r\u00fc<\/span><span class=\"result-term\">k1<\/span><\/span><span class=\"field-value\" id=\"result-2\"><\/span><button type=\"button\" class=\"copy-result\" data-tooltip=\"Sonucu kopyala\" aria-label=\"Sonucu kopyala\"><span class=\"copy-icon icon icon-document-copy\" aria-hidden=\"true\"><\/span><\/button><\/div><div class=\"field-result\" id=\"output-3\"><span class=\"field-title\"><span>2. bile\u015fi\u011fin al\u0131konma fakt\u00f6r\u00fc<\/span><span class=\"result-term\">k2<\/span><\/span><span class=\"field-value\" id=\"result-3\"><\/span><button type=\"button\" class=\"copy-result\" data-tooltip=\"Sonucu kopyala\" aria-label=\"Sonucu kopyala\"><span class=\"copy-icon icon icon-document-copy\" aria-hidden=\"true\"><\/span><\/button><\/div><div class=\"field-result is-column\" id=\"output-4\"><span class=\"field-title\"><span>Hesaplama Ayr\u0131nt\u0131lar\u0131<\/span><\/span><span class=\"field-value\" id=\"result-4\"><\/span><button type=\"button\" class=\"copy-result\" data-tooltip=\"Sonucu kopyala\" aria-label=\"Sonucu kopyala\"><span class=\"copy-icon icon icon-document-copy\" aria-hidden=\"true\"><\/span><\/button><\/div><\/div><div class=\"result-actions\"><button type=\"button\" class=\"copy-link\"><svg viewBox=\"0 0 20 20\" fill=\"currentColor\" aria-hidden=\"true\"><path d=\"M12.232 4.232a2.5 2.5 0 0 1 3.536 3.536l-1.225 1.224a.75.75 0 0 0 1.061 1.06l1.224-1.224a4 4 0 0 0-5.656-5.656l-3 3a4 4 0 0 0 .225 5.865.75.75 0 0 0 .977-1.138 2.5 2.5 0 0 1-.142-3.667l3-3Z\"\/><path d=\"M11.603 7.963a.75.75 0 0 0-.977 1.138 2.5 2.5 0 0 1 .142 3.667l-3 3a2.5 2.5 0 0 1-3.536-3.536l1.225-1.224a.75.75 0 0 0-1.061-1.06l-1.224 1.224a4 4 0 1 0 5.656 5.656l3-3a4 4 0 0 0-.225-5.865Z\"\/><\/svg><span class=\"copy-link__text\">Bu hesaplaman\u0131n ba\u011flant\u0131s\u0131n\u0131 kopyala<\/span><\/button><span class=\"sr-only\" role=\"status\" id=\"copy-link-status\"><\/span><\/div><\/div><a href=\"#respond\" class=\"hidden transition-opacity duration-300 opacity-0 w-50 text-sm justify-center items-center gap-2 px-4 py-2 rounded bg-gray-200 text-gray-700 hover:bg-gray-300\" id=\"leave-comment\"><span class=\"icon icon-comments\" aria-hidden=\"true\"><\/span>Yorum b\u0131rak<\/a><\/form><\/section><section id=\"calc-reactions\" class=\"not-prose hidden my-12 bg-gradient-to-r from-primary-50 to-gray-50 border border-indigo-100 rounded-xl px-6 py-4 shadow-sm\" data-post=\"3948\" aria-live=\"polite\" aria-labelledby=\"calc-reactions-title\"><p id=\"calc-reactions-title\" class=\"text-sm text-gray-500 text-center mb-2\">Bu sonu\u00e7 hakk\u0131nda ne d\u00fc\u015f\u00fcn\u00fcyorsunuz?<\/p><div role=\"group\" aria-labelledby=\"calc-reactions-title\" class=\"grid grid-cols-3 sm:flex sm:flex-row sm:justify-around gap-4 sm:items-center sm:flex-wrap\"><button type=\"button\" aria-pressed=\"false\" class=\"reaction-btn flex flex-col items-center gap-1 text-sm text-gray-700 hover:text-primary-600 cursor-pointer transition-transform hover:scale-105\" data-reaction=\"like\"><span class=\"reaction-count\" data-reaction-count=\"like\">0<\/span><span class=\"reaction text-3xl\" aria-hidden=\"true\">\ud83d\ude00 <\/span><span class=\"reaction-description font-medium\">Be\u011fendim <\/span><\/button><button type=\"button\" aria-pressed=\"false\" class=\"reaction-btn flex flex-col items-center gap-1 text-sm text-gray-700 hover:text-primary-600 cursor-pointer transition-transform hover:scale-105\" data-reaction=\"helpful\"><span class=\"reaction-count\" data-reaction-count=\"helpful\">0<\/span><span class=\"reaction text-3xl\" aria-hidden=\"true\">\ud83d\udca1 <\/span><span class=\"reaction-description font-medium\">Faydal\u0131 <\/span><\/button><button type=\"button\" aria-pressed=\"false\" class=\"reaction-btn flex flex-col items-center gap-1 text-sm text-gray-700 hover:text-primary-600 cursor-pointer transition-transform hover:scale-105\" data-reaction=\"confused\"><span class=\"reaction-count\" data-reaction-count=\"confused\">0<\/span><span class=\"reaction text-3xl\" aria-hidden=\"true\">\ud83d\ude15 <\/span><span class=\"reaction-description font-medium\">Kafam kar\u0131\u015ft\u0131 <\/span><\/button><button type=\"button\" aria-pressed=\"false\" class=\"reaction-btn flex flex-col items-center gap-1 text-sm text-gray-700 hover:text-primary-600 cursor-pointer transition-transform hover:scale-105\" data-reaction=\"disappointed\"><span class=\"reaction-count\" data-reaction-count=\"disappointed\">0<\/span><span class=\"reaction text-3xl\" aria-hidden=\"true\">\ud83d\ude1e <\/span><span class=\"reaction-description font-medium\">Hayal k\u0131r\u0131kl\u0131\u011f\u0131 <\/span><\/button><button type=\"button\" aria-pressed=\"false\" class=\"reaction-btn flex flex-col items-center gap-1 text-sm text-gray-700 hover:text-primary-600 cursor-pointer transition-transform hover:scale-105\" data-reaction=\"inaccurate\"><span class=\"reaction-count\" data-reaction-count=\"inaccurate\">0<\/span><span class=\"reaction text-3xl\" aria-hidden=\"true\">\u274c <\/span><span class=\"reaction-description font-medium\">Hatal\u0131 <\/span><\/button><\/div><div id=\"reaction-message\" class=\"hidden mt-8 rounded-md border border-primary-100 bg-white\/50 backdrop-blur-sm px-4 py-2 text-sm text-center shadow-sm\"><\/div><\/section><div class=\"calcdash-ad not-prose calcdash-ad--calc_result calcdash-ad--in-article\"><ins class=\"adsbygoogle\" style=\"display:block\" data-ad-client=\"ca-pub-1721569815777345\" data-ad-slot=\"6317458308\" data-ad-layout=\"in-article\" data-ad-format=\"fluid\"><\/ins><script>(adsbygoogle = window.adsbygoogle || []).push({});<\/script><\/div>\n<style id=\"wpcoderpro-inline-1\">.formula-box {\r\n\tborder-bottom: none !important;\r\n}<\/style><section class=\"formula-box\" aria-labelledby=\"formula-title\">\r\n\t<div id=\"formula-title\" class=\"not-prose font-display mb-4\"><span class=\"icon icon-formula\" aria-hidden=\"true\"><\/span><h2>Kromatografi Se\u00e7icilik Fakt\u00f6r\u00fc Form\u00fcl\u00fc<\/h2><\/div>\r\n\t<figure class=\"not-prose formula\">\r\n\t\t<figcaption class=\"formula-title\">Form\u00fcl<\/figcaption>\r\n\t\t<div class=\"text-base text-gray-800\" id=\"formula\">\n\\[ \\alpha = \\frac{k_2}{k_1} = \\frac{t_{R2} - t_0}{t_{R1} - t_0} \\]\n<\/div>\r\n\t<\/figure>\r\n\t<\/p>\n<p><strong>Buradaki terimler:<\/strong><\/p>\n<ul>\n<li>\\(t_{R1}\\) \u2014 \u00f6nce el\u00fce olan bile\u015fi\u011fin retansiyon s\u00fcresi (dk veya sn)<\/li>\n<li>\\(t_{R2}\\) \u2014 sonra el\u00fce olan bile\u015fi\u011fin retansiyon s\u00fcresi (dk veya sn)<\/li>\n<li>\\(t_0\\) \u2014 \u00f6l\u00fc zaman (ge\u00e7irme zaman\u0131), yani tutulmayan bir bile\u015fi\u011fin kolonda ge\u00e7irdi\u011fi s\u00fcre<\/li>\n<li>\\(k_1, k_2\\) \u2014 iki bile\u015fi\u011fin al\u0131konma fakt\u00f6rleri, \\(k = (t_R - t_0)\/t_0\\)<\/li>\n<\/ul>\n<p>Form\u00fcl, her bile\u015fi\u011fin \u00f6l\u00fc zamana g\u00f6re ne kadar daha uzun tutuldu\u011funu kar\u015f\u0131la\u015ft\u0131r\u0131r. \\(t_0\\) oran i\u00e7inde sadele\u015fti\u011fi i\u00e7in \\(\\alpha\\), yaln\u0131zca iki analitin sabit faz ve hareketli fazla ne kadar farkl\u0131 etkile\u015fti\u011fine ba\u011fl\u0131d\u0131r.<\/p>\n<p>\r\n<\/section>\n<style id=\"wpcoderpro-inline-2\">.calculation-box {\r\n\tborder-bottom: none !important;\r\n}<\/style><section id=\"calculation\" class=\"calculation-box\" aria-labelledby=\"calculation-title\">\r\n\t<div id=\"calculation-title\" class=\"not-prose font-display\"><span class=\"icon icon-unordered-list\" aria-hidden=\"true\"><\/span><h2>Kromatografi Se\u00e7icilik Fakt\u00f6r\u00fc \u2013 Hesaplama \u00d6rne\u011fi<\/h2><\/div>\r\n\r\n<\/p>\n<p><strong>\u00d6rnek:<\/strong> izokratik bir HPLC \u00e7al\u0131\u015fmas\u0131nda ilk pik 4,2 dakikada, ikinci pik 6,8 dakikada, \u00e7\u00f6z\u00fcc\u00fc cephesi (tutulmayan i\u015faretleyici) ise 1,5 dakikada g\u00f6r\u00fcn\u00fcyor.<\/p>\n<p>\\[ k_1 = \\frac{4.2 - 1.5}{1.5} = 1.8000 \\qquad k_2 = \\frac{6.8 - 1.5}{1.5} = 3.5333 \\]<\/p>\n<p>\\[ \\alpha = \\frac{3.5333}{1.8000} = 1.9630 \\]<\/p>\n<p><strong>Sonu\u00e7:<\/strong> se\u00e7icilik fakt\u00f6r\u00fc <strong>\u03b1 = 1,963<\/strong>, al\u0131konma fakt\u00f6rleri ise k\u2081 = 1,80 ve k\u2082 = 3,53. \u03b1'n\u0131n 2'ye yak\u0131n olmas\u0131, bu \u00e7iftin iyi ayr\u0131ld\u0131\u011f\u0131 ve makul verimlilikte bir kolonla taban ayr\u0131m\u0131na kolayca ula\u015f\u0131laca\u011f\u0131 anlam\u0131na gelir.<\/p>\n<p>\n\r\n<\/section>\n<h2>Hesaplay\u0131c\u0131 Nas\u0131l Kullan\u0131l\u0131r?<\/h2>\n<ol>\n<li><strong>Bile\u015fik 1'in retansiyon s\u00fcresini<\/strong> girin \u2014 enjeksiyon an\u0131ndan \u00f6l\u00e7\u00fclen, \u00f6nce el\u00fce olan pikin s\u00fcresi. Yukar\u0131daki \u00f6rne\u011fi yeniden elde etmek i\u00e7in varsay\u0131lan 4,2 de\u011ferini kullan\u0131n.<\/li>\n<li><strong>Bile\u015fik 2'nin retansiyon s\u00fcresini<\/strong> girin \u2014 sonra el\u00fce olan pik (varsay\u0131lan 6,8 dk). Daha b\u00fcy\u00fck de\u011feri yanl\u0131\u015fl\u0131kla ilk alana girerseniz, hesaplay\u0131c\u0131 ikisini otomatik olarak yer de\u011fi\u015ftirir; b\u00f6ylece \u03b1 her zaman \u2265 1 olur.<\/li>\n<li><strong>\u00d6l\u00fc zaman\u0131<\/strong> \\(t_0\\) girin \u2014 kolonun ge\u00e7irme zaman\u0131; genellikle tutulmayan bir i\u015faretleyiciden veya \u00e7\u00f6z\u00fcc\u00fc cephesinden okunur (varsay\u0131lan 1,5).<\/li>\n<li>Her alan i\u00e7in birim se\u00e7in (<strong>dk<\/strong> veya <strong>sn<\/strong>). Sonucun ge\u00e7erli olmas\u0131 i\u00e7in \u00fc\u00e7 s\u00fcrenin de ayn\u0131 birimde olmas\u0131 gerekir; hesaplay\u0131c\u0131 her \u015feyi i\u00e7sel olarak dakikaya \u00e7evirir.<\/li>\n<li>Sonu\u00e7larda <strong>se\u00e7icilik fakt\u00f6r\u00fcn\u00fc<\/strong>, iki al\u0131konma fakt\u00f6r\u00fcn\u00fc ve ay\u0131rma kalitesine ili\u015fkin yorumu okuyun.<\/li>\n<\/ol>\n<h2>Se\u00e7icilik Fakt\u00f6r\u00fc Nedir?<\/h2>\n<p>Se\u00e7icilik fakt\u00f6r\u00fc \u03b1 (ay\u0131rt etme fakt\u00f6r\u00fc olarak da bilinir), bir kromatografik kolonun iki bile\u015fi\u011fi birbirinden ne kadar farkl\u0131 tuttu\u011funu \u00f6l\u00e7er. Sonra ve \u00f6nce el\u00fce olan piklerin al\u0131konma fakt\u00f6rlerinin oran\u0131d\u0131r. \u03b1'n\u0131n tam 1 olmas\u0131, iki bile\u015fi\u011fin sabit fazda ayn\u0131 oranda zaman ge\u00e7irdi\u011fi ve tek bir pik olarak birlikte el\u00fce oldu\u011fu anlam\u0131na gelir \u2014 kolon verimlili\u011fini ne kadar art\u0131r\u0131rsan\u0131z art\u0131r\u0131n bunlar\u0131 ay\u0131ramazs\u0131n\u0131z. \u03b1, 1'in \u00fczerinde ne kadar y\u00fckselirse, kolon bu \u00e7ifti o kadar iyi ay\u0131rt eder.<\/p>\n<p>Se\u00e7icili\u011fi kolon verimlili\u011fi ve pik geni\u015fli\u011fiyle harmanlayan \u00e7\u00f6z\u00fcn\u00fcrl\u00fckten farkl\u0131 olarak \u03b1 yaln\u0131zca termodinamik bir b\u00fcy\u00fckl\u00fckt\u00fcr. Sistemin kimyas\u0131 belirler: sabit faz, hareketli faz\u0131n bile\u015fimi, pH ve s\u0131cakl\u0131k. Ak\u0131\u015f h\u0131z\u0131n\u0131, kolon uzunlu\u011funu veya partik\u00fcl boyutunu de\u011fi\u015ftirmek \u03b1'y\u0131 de\u011fi\u015ftirmez; yaln\u0131zca mevcut se\u00e7icilikten ne kadar verimli yararlan\u0131ld\u0131\u011f\u0131n\u0131 etkiler. Bu y\u00fczden iki pik bir t\u00fcrl\u00fc ayr\u0131lm\u0131yorsa do\u011fru \u00e7\u00f6z\u00fcm genellikle daha uzun bir kolon de\u011fil, kimyasal bir de\u011fi\u015fikliktir (farkl\u0131 bir sabit faz, daha g\u00fc\u00e7l\u00fc veya daha zay\u0131f bir \u00e7\u00f6z\u00fcc\u00fc, ayarlanm\u0131\u015f bir pH).<\/p>\n<p>Sonu\u00e7lar\u0131 yorumlamak i\u00e7in pratik bir k\u0131lavuz: 1,05'in alt\u0131ndaki \u03b1, piklerin pratikte birlikte el\u00fce oldu\u011fu anlam\u0131na gelir; 1,05 ile 1,2 aras\u0131ndaki \u03b1 \u00e7ok verimli bir kolon ve titiz bir optimizasyon gerektirir; 1,2 ile 2 aras\u0131ndaki \u03b1 normalde taban ayr\u0131m\u0131 verir; 2'nin \u00fczerindeki de\u011ferler ise rahat\u00e7a ayr\u0131lm\u0131\u015f bir \u00e7ifte i\u015faret eder, hatta bazen daha k\u0131sa ve h\u0131zl\u0131 bir analiz yap\u0131lmas\u0131na izin verir. Al\u0131konma fakt\u00f6rleri k\u2081 ve k\u2082'nin ideal aral\u0131\u011f\u0131 1 ile 10 aras\u0131d\u0131r \u2014 d\u00fc\u015f\u00fck k de\u011ferleri pikleri \u00e7\u00f6z\u00fcc\u00fc cephesine \u00e7ok yakla\u015ft\u0131r\u0131r, y\u00fcksek de\u011ferler ise analiz s\u00fcresini uzat\u0131r ve pikleri geni\u015fletir.<\/p>\n<p>Se\u00e7icilik fakt\u00f6r\u00fc do\u011frudan her bile\u015fi\u011fin <a href=\"https:\/\/wpcalc.com\/tr\/kimya\/kromatografi-tutma-faktoru-hesaplayici\/\">al\u0131konma fakt\u00f6r\u00fcne<\/a> ba\u011flan\u0131r ve <a href=\"https:\/\/wpcalc.com\/en\/chemistry\/chromatography-theoretical-plates-calculator\/\">teorik plaka say\u0131s\u0131yla<\/a> birlikte bu \u00e7ift i\u00e7in elde edilebilecek \u00e7\u00f6z\u00fcn\u00fcrl\u00fc\u011f\u00fc belirler. B\u00f6l\u00fcnme temelli ay\u0131rmalarda \u03b1, sonu\u00e7ta iki analitin iki faz aras\u0131ndaki <a href=\"https:\/\/wpcalc.com\/tr\/kimya\/bolusme-katsayisi-hesaplama\/\">b\u00f6l\u00fcnme katsay\u0131lar\u0131ndaki<\/a> fark\u0131 yans\u0131t\u0131r.<\/p>\n<h2>Varsay\u0131mlar, S\u0131n\u0131rlamalar ve Pratik \u00d6neriler<\/h2>\n<ul>\n<li><strong>Ayn\u0131 ko\u015fu ve ayn\u0131 birim:<\/strong> iki retansiyon s\u00fcresi de \u00f6l\u00fc zamanla ayn\u0131 kromatografik ko\u015fudan gelmeli ve ayn\u0131 birimle ifade edilmelidir (hesaplay\u0131c\u0131 dk\/sn d\u00f6n\u00fc\u015f\u00fcm\u00fcn\u00fc sizin yerinize yapar, ancak birbirine uymayan de\u011ferlerle birim kar\u0131\u015ft\u0131rmak anlams\u0131z bir oran verir).<\/li>\n<li><strong>\u0130zokratik ko\u015fullar:<\/strong> form\u00fcl, el\u00fcsyon g\u00fcc\u00fcn\u00fcn sabit oldu\u011funu varsayar. Gradiyan el\u00fcsyon alt\u0131nda retansiyon s\u00fcreleri gradiyan program\u0131na ba\u011fl\u0131d\u0131r ve klasik \u03b1 basit anlam\u0131n\u0131 yitirir.<\/li>\n<li><strong>Do\u011fru \u00f6l\u00fc zaman:<\/strong> \u03b1, \\(t_0\\) de\u011ferine duyarl\u0131d\u0131r. Sisteminiz i\u00e7in ger\u00e7ekten tutulmayan bir i\u015faretleyiciyle \u00f6l\u00e7\u00fcn; yanl\u0131\u015f bir ge\u00e7irme zaman\u0131 hem k de\u011ferlerini \u00e7arp\u0131t\u0131r hem de piklerin k de\u011ferleri \u00e7ok farkl\u0131ysa oran\u0131 bozar.<\/li>\n<li><strong>\u0130ki pik de tutulmu\u015f olmal\u0131:<\/strong> her retansiyon s\u00fcresi \\(t_0\\) de\u011ferini a\u015fmal\u0131d\u0131r. \u00d6nceki pik \u00f6l\u00fc zamandan \u00f6nce veya tam o anda el\u00fce oluyorsa al\u0131konma fakt\u00f6r\u00fc s\u0131f\u0131r ya da negatif olur ve \u03b1 tan\u0131ms\u0131zd\u0131r.<\/li>\n<li><strong>\u03b1, \u00e7\u00f6z\u00fcn\u00fcrl\u00fck de\u011fildir:<\/strong> b\u00fcy\u00fck bir \u03b1, dar ve iyi ayr\u0131lm\u0131\u015f pikleri garanti etmez \u2014 pik geni\u015fli\u011fi ve kolon verimlili\u011fi de \u00f6nemlidir. Kimyas\u0131\u011f\u0131 \u03b1 ile, donan\u0131m\u0131 ise plaka say\u0131lar\u0131 veya \u00e7\u00f6z\u00fcn\u00fcrl\u00fck form\u00fclleriyle de\u011ferlendirin.<\/li>\n<li><strong>Optimizasyon stratejisi:<\/strong> y\u00f6ntem geli\u015ftirmede \u00f6nce sa\u011fl\u0131kl\u0131 bir \u03b1 hedefleyin (sabit faz\u0131, organik modifikat\u00f6r\u00fc veya pH'\u0131 de\u011fi\u015ftirin), ard\u0131ndan verimlili\u011fi ve h\u0131z\u0131 ince ayarlay\u0131n. Yaln\u0131zca ak\u0131\u015f h\u0131z\u0131n\u0131 de\u011fi\u015ftirmek \u03b1'y\u0131 hi\u00e7 k\u0131p\u0131rdatmaz.<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>\u0130ki bile\u015fi\u011fin retansiyon s\u00fcrelerini ve kolonun \u00f6l\u00fc zaman\u0131n\u0131 girin; se\u00e7icilik fakt\u00f6r\u00fcn\u00fc (\u03b1) ve iki al\u0131konma fakt\u00f6r\u00fcn\u00fc hesaplay\u0131n, ayr\u0131ca kolonun bu \u00e7ifti ne kadar iyi ay\u0131rd\u0131\u011f\u0131n\u0131 g\u00f6r\u00fcn.<\/p>\n","protected":false},"author":3168,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[3],"tags":[68],"class_list":["post-3948","post","type-post","status-publish","format-standard","hentry","category-kimya","tag-spektrofotometri"],"acf":[],"_links":{"self":[{"href":"https:\/\/wpcalc.com\/tr\/wp-json\/wp\/v2\/posts\/3948","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wpcalc.com\/tr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wpcalc.com\/tr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wpcalc.com\/tr\/wp-json\/wp\/v2\/users\/3168"}],"replies":[{"embeddable":true,"href":"https:\/\/wpcalc.com\/tr\/wp-json\/wp\/v2\/comments?post=3948"}],"version-history":[{"count":0,"href":"https:\/\/wpcalc.com\/tr\/wp-json\/wp\/v2\/posts\/3948\/revisions"}],"wp:attachment":[{"href":"https:\/\/wpcalc.com\/tr\/wp-json\/wp\/v2\/media?parent=3948"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wpcalc.com\/tr\/wp-json\/wp\/v2\/categories?post=3948"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wpcalc.com\/tr\/wp-json\/wp\/v2\/tags?post=3948"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}