{"id":3963,"date":"2026-10-14T19:41:17","date_gmt":"2026-10-14T18:41:17","guid":{"rendered":"https:\/\/wpcalc.com\/tr\/kimya\/dna-erime-sicakligi-hesaplayici\/"},"modified":"2026-10-14T19:41:17","modified_gmt":"2026-10-14T18:41:17","slug":"dna-erime-sicakligi-hesaplayici","status":"publish","type":"post","link":"https:\/\/wpcalc.com\/tr\/kimya\/dna-erime-sicakligi-hesaplayici\/","title":{"rendered":"DNA Erime S\u0131cakl\u0131\u011f\u0131 Hesaplama Arac\u0131"},"content":{"rendered":"<section class=\"not-prose calculator-box\" aria-labelledby=\"calculator-title\"><div class=\"flex items-baseline sm:items-center justify-between gap-2 sm:gap-3\"><div class=\"flex flex-col sm:flex-row sm:items-center gap-2\"><span class=\"icon icon-calculator text-primary-700! text-base! dark:text-primary-300!\" aria-hidden=\"true\"><\/span><h2 id=\"calculator-title\" class=\"text-lg font-display font-bold\">DNA Oligon\u00fckleotidinin Erime S\u0131cakl\u0131\u011f\u0131n\u0131 Tahmin Edin<\/h2><\/div><div class=\"calcdash-favorite group\">\n  <button type=\"button\" class=\"favorite\" id=\"favorite\" data-favorite-id=\"3963\" data-favorite-title=\"DNA Erime S\u0131cakl\u0131\u011f\u0131\" data-favorite-url=\"https:\/\/wpcalc.com\/tr\/kimya\/dna-erime-sicakligi-hesaplayici\/\" data-favorite-excerpt=\"Bir DNA oligon\u00fckleotidinin erime s\u0131cakl\u0131\u011f\u0131n\u0131 (Tm), dizilimi ve tuz konsantrasyonundan tahmin edin. Tek zinciri (A, C, G, T) ve Na\u207a konsantrasyonunu girin; Tm ve GC i\u00e7eri\u011fini an\u0131nda g\u00f6r\u00fcn.\" aria-label=\"Favorilere ekle\" data-favorite-icon=\"icon icon-biology\">\n    <span class=\"icon icon-shape-star-empty\" aria-hidden=\"true\"><\/span>\n  <\/button>\n  <div class=\"calcdash-favorite__tip\" aria-hidden=\"true\">\n   <span class=\"favorite-tooltip\" id=\"favorite-tooltip\"><\/span>\n  <\/div>\n  <span class=\"sr-only\" role=\"status\" id=\"favorite-status\"><\/span>\n<\/div><\/div><form action=\"https:\/\/wpcalc.com\/tr\/kimya\/dna-erime-sicakligi-hesaplayici\/\" method=\"POST\" class=\"calculator\" id=\"calculator-3963\" data-post=\"3963\"><fieldset class=\"fieldset-input is-1\"><legend class=\"sr-only\">Giri\u015f alanlar\u0131<\/legend><div class=\"field is-single\" id=\"input-1\"><label for=\"field-1\">DNA Dizisi (5\u2032\u21923\u2032)<\/label><textarea id=\"field-1\" name=\"dna_dizisi_53\">ATGCATGCGATTACA<\/textarea><\/div><div class=\"field has-term\" id=\"input-2\"><label for=\"field-2\">Tuz (Na\u207a) Konsantrasyonu<\/label><div class=\"term\">[Na\u207a]<\/div>  <div class=\"absolute bottom-0 left-16 top-8 w-2 h-0.5 bg-blue-500\"><\/div><input type=\"text\" name=\"tuz_na_konsantrasyonu\" id=\"field-2\" aria-describedby=\"calc-unit-2 calc-info-2\" data-number inputmode=\"decimal\" autocomplete=\"off\" spellcheck=\"false\" placeholder=\"e.g. 50\" min=\"0\" value=\"50\"\/><div class=\"unit\" id=\"calc-unit-2\">mM<\/div><small id=\"calc-info-2\">Toplam monovalent tuz konsantrasyonu. 14 baz ve daha uzun diziler i\u00e7in tuz d\u00fczeltmeli form\u00fclde kullan\u0131l\u0131r; daha k\u0131sa oligolarda uygulanan Wallace kural\u0131 bu de\u011feri kullanmaz.<\/small><\/div><\/fieldset><div class=\"buttons\"><button type=\"submit\" data-text=\"Yeniden hesapla\" id=\"calculate-button\" data-post=\"3963\">Hesapla<\/button><button type=\"reset\">S\u0131f\u0131rla<\/button><\/div><div class=\"field is-checkbox hidden!\" id=\"field-auto-calc\"><input type=\"checkbox\" id=\"auto-calc\"><label for=\"auto-calc\">Otomatik hesapla<\/label><small>Etkinle\u015ftirilirse, herhangi bir de\u011feri de\u011fi\u015ftirdi\u011finizde sonu\u00e7 otomatik olarak g\u00fcncellenir.<\/small><\/div><div class=\"fieldset-result is-hidden\" aria-labelledby=\"result-title\" aria-live=\"polite\" role=\"region\"> <h3 class=\"result-title bg-gradient-to-r from-primary-50 to-gray-50 dark:from-primary-900 dark:to-gray-800\" id=\"result-title\"><span class=\"icon icon-s-pulse\" aria-hidden=\"true\"><\/span> Erime S\u0131cakl\u0131\u011f\u0131 ve GC \u0130\u00e7eri\u011fi<\/h3><div class=\"result-box\"><div class=\"field-result\" id=\"output-1\"><span class=\"field-title\"><span>Erime S\u0131cakl\u0131\u011f\u0131 (Tm)<\/span><span class=\"result-term\">Tm<\/span><\/span><span class=\"field-value\" id=\"result-1\" data-unit=\"\u00b0C\"><\/span><button type=\"button\" class=\"copy-result\" data-tooltip=\"Sonucu kopyala\" aria-label=\"Sonucu kopyala\"><span class=\"copy-icon icon icon-document-copy\" aria-hidden=\"true\"><\/span><\/button><\/div><div class=\"field-result\" id=\"output-2\"><span class=\"field-title\"><span>GC \u0130\u00e7eri\u011fi<\/span><span class=\"result-term\">GC<\/span><\/span><span class=\"field-value\" id=\"result-2\" data-unit=\"%\"><\/span><button type=\"button\" class=\"copy-result\" data-tooltip=\"Sonucu kopyala\" aria-label=\"Sonucu kopyala\"><span class=\"copy-icon icon icon-document-copy\" aria-hidden=\"true\"><\/span><\/button><\/div><div class=\"field-result is-column\" id=\"output-3\"><span class=\"field-title\"><span>Hesaplama Ayr\u0131nt\u0131lar\u0131<\/span><\/span><span class=\"field-value\" id=\"result-3\"><\/span><button type=\"button\" class=\"copy-result\" data-tooltip=\"Sonucu kopyala\" aria-label=\"Sonucu kopyala\"><span class=\"copy-icon icon icon-document-copy\" aria-hidden=\"true\"><\/span><\/button><\/div><div class=\"field-result is-column\" id=\"output-4\"><span class=\"field-title\"><span>Form\u00fcller ve Varsay\u0131mlar Hakk\u0131nda<\/span><\/span><span class=\"field-value\" id=\"result-4\"><\/span><button type=\"button\" class=\"copy-result\" data-tooltip=\"Sonucu kopyala\" aria-label=\"Sonucu kopyala\"><span class=\"copy-icon icon icon-document-copy\" aria-hidden=\"true\"><\/span><\/button><\/div><\/div><div class=\"result-actions\"><button type=\"button\" class=\"copy-link\"><svg viewBox=\"0 0 20 20\" fill=\"currentColor\" aria-hidden=\"true\"><path d=\"M12.232 4.232a2.5 2.5 0 0 1 3.536 3.536l-1.225 1.224a.75.75 0 0 0 1.061 1.06l1.224-1.224a4 4 0 0 0-5.656-5.656l-3 3a4 4 0 0 0 .225 5.865.75.75 0 0 0 .977-1.138 2.5 2.5 0 0 1-.142-3.667l3-3Z\"\/><path d=\"M11.603 7.963a.75.75 0 0 0-.977 1.138 2.5 2.5 0 0 1 .142 3.667l-3 3a2.5 2.5 0 0 1-3.536-3.536l1.225-1.224a.75.75 0 0 0-1.061-1.06l-1.224 1.224a4 4 0 1 0 5.656 5.656l3-3a4 4 0 0 0-.225-5.865Z\"\/><\/svg><span class=\"copy-link__text\">Bu hesaplaman\u0131n ba\u011flant\u0131s\u0131n\u0131 kopyala<\/span><\/button><span class=\"sr-only\" role=\"status\" id=\"copy-link-status\"><\/span><\/div><\/div><a href=\"#respond\" class=\"hidden transition-opacity duration-300 opacity-0 w-50 text-sm justify-center items-center gap-2 px-4 py-2 rounded bg-gray-200 text-gray-700 hover:bg-gray-300\" id=\"leave-comment\"><span class=\"icon icon-comments\" aria-hidden=\"true\"><\/span>Yorum b\u0131rak<\/a><\/form><\/section><section id=\"calc-reactions\" class=\"not-prose hidden my-12 bg-gradient-to-r from-primary-50 to-gray-50 border border-indigo-100 rounded-xl px-6 py-4 shadow-sm\" data-post=\"3963\" aria-live=\"polite\" aria-labelledby=\"calc-reactions-title\"><p id=\"calc-reactions-title\" class=\"text-sm text-gray-500 text-center mb-2\">Bu sonu\u00e7 hakk\u0131nda ne d\u00fc\u015f\u00fcn\u00fcyorsunuz?<\/p><div role=\"group\" aria-labelledby=\"calc-reactions-title\" class=\"grid grid-cols-3 sm:flex sm:flex-row sm:justify-around gap-4 sm:items-center sm:flex-wrap\"><button type=\"button\" aria-pressed=\"false\" class=\"reaction-btn flex flex-col items-center gap-1 text-sm text-gray-700 hover:text-primary-600 cursor-pointer transition-transform hover:scale-105\" data-reaction=\"like\"><span class=\"reaction-count\" data-reaction-count=\"like\">0<\/span><span class=\"reaction text-3xl\" aria-hidden=\"true\">\ud83d\ude00 <\/span><span class=\"reaction-description font-medium\">Be\u011fendim <\/span><\/button><button type=\"button\" aria-pressed=\"false\" class=\"reaction-btn flex flex-col items-center gap-1 text-sm text-gray-700 hover:text-primary-600 cursor-pointer transition-transform hover:scale-105\" data-reaction=\"helpful\"><span class=\"reaction-count\" data-reaction-count=\"helpful\">0<\/span><span class=\"reaction text-3xl\" aria-hidden=\"true\">\ud83d\udca1 <\/span><span class=\"reaction-description font-medium\">Faydal\u0131 <\/span><\/button><button type=\"button\" aria-pressed=\"false\" class=\"reaction-btn flex flex-col items-center gap-1 text-sm text-gray-700 hover:text-primary-600 cursor-pointer transition-transform hover:scale-105\" data-reaction=\"confused\"><span class=\"reaction-count\" data-reaction-count=\"confused\">0<\/span><span class=\"reaction text-3xl\" aria-hidden=\"true\">\ud83d\ude15 <\/span><span class=\"reaction-description font-medium\">Kafam kar\u0131\u015ft\u0131 <\/span><\/button><button type=\"button\" aria-pressed=\"false\" class=\"reaction-btn flex flex-col items-center gap-1 text-sm text-gray-700 hover:text-primary-600 cursor-pointer transition-transform hover:scale-105\" data-reaction=\"disappointed\"><span class=\"reaction-count\" data-reaction-count=\"disappointed\">0<\/span><span class=\"reaction text-3xl\" aria-hidden=\"true\">\ud83d\ude1e <\/span><span class=\"reaction-description font-medium\">Hayal k\u0131r\u0131kl\u0131\u011f\u0131 <\/span><\/button><button type=\"button\" aria-pressed=\"false\" class=\"reaction-btn flex flex-col items-center gap-1 text-sm text-gray-700 hover:text-primary-600 cursor-pointer transition-transform hover:scale-105\" data-reaction=\"inaccurate\"><span class=\"reaction-count\" data-reaction-count=\"inaccurate\">0<\/span><span class=\"reaction text-3xl\" aria-hidden=\"true\">\u274c <\/span><span class=\"reaction-description font-medium\">Hatal\u0131 <\/span><\/button><\/div><div id=\"reaction-message\" class=\"hidden mt-8 rounded-md border border-primary-100 bg-white\/50 backdrop-blur-sm px-4 py-2 text-sm text-center shadow-sm\"><\/div><\/section><div class=\"calcdash-ad not-prose calcdash-ad--calc_result calcdash-ad--in-article\"><ins class=\"adsbygoogle\" style=\"display:block\" data-ad-client=\"ca-pub-1721569815777345\" data-ad-slot=\"6317458308\" data-ad-layout=\"in-article\" data-ad-format=\"fluid\"><\/ins><script>(adsbygoogle = window.adsbygoogle || []).push({});<\/script><\/div>\n<style id=\"wpcoderpro-inline-1\">.formula-box {\r\n\tborder-bottom: none !important;\r\n}<\/style><section class=\"formula-box\" aria-labelledby=\"formula-title\">\r\n\t<div id=\"formula-title\" class=\"not-prose font-display mb-4\"><span class=\"icon icon-formula\" aria-hidden=\"true\"><\/span><h2>DNA Erime S\u0131cakl\u0131\u011f\u0131 Form\u00fcl\u00fc<\/h2><\/div>\r\n\t<figure class=\"not-prose formula\">\r\n\t\t<figcaption class=\"formula-title\">Form\u00fcl<\/figcaption>\r\n\t\t<div class=\"text-base text-gray-800\" id=\"formula\">\n\\[ T_m = 2\\,(A+T) + 4\\,(G+C) \\\\ T_m = 100.5 + 41\\,\\frac{G+C}{n} - \\frac{820}{n} + 16.6\\,\\log_{10}[\\mathrm{Na^+}] \\]\n<\/div>\r\n\t<\/figure>\r\n\t<\/p>\n<p><strong>Burada:<\/strong><\/p>\n<ul>\n<li>\\(A, T, G, C\\) \u2014 dizilimdeki adenin, timin, guanin ve sitozin baz\u0131lar\u0131n\u0131n say\u0131s\u0131<\/li>\n<li>\\(n\\) \u2014 toplam baz say\u0131s\u0131 (dizilim uzunlu\u011fu)<\/li>\n<li>\\([\\mathrm{Na^+}]\\) \u2014 mol\/L cinsinden toplam monovalent tuz konsantrasyonu<\/li>\n<\/ul>\n<p>\u0130lk denklem, 13 baza kadar k\u0131sa oligon\u00fckleotitler i\u00e7in kullan\u0131lan Wallace kural\u0131d\u0131r; ikincisi ise tuz d\u00fczeltmeli form\u00fcld\u00fcr (Howley ve ark., 1979) ve 14 bazdan itibaren otomatik olarak uygulan\u0131r. Hesaplay\u0131c\u0131, sizin i\u00e7in uygun form\u00fcl\u00fc kendisi se\u00e7er.<\/p>\n<p>\r\n<\/section>\n<style id=\"wpcoderpro-inline-2\">.calculation-box {\r\n\tborder-bottom: none !important;\r\n}<\/style><section id=\"calculation\" class=\"calculation-box\" aria-labelledby=\"calculation-title\">\r\n\t<div id=\"calculation-title\" class=\"not-prose font-display\"><span class=\"icon icon-unordered-list\" aria-hidden=\"true\"><\/span><h2>DNA Erime S\u0131cakl\u0131\u011f\u0131 \u2013 Hesaplama \u00d6rne\u011fi<\/h2><\/div>\r\n\r\n<\/p>\n<p><strong>\u00d6rnek:<\/strong> ATGCATGCGATTACA dizilimine sahip, 15 baz uzunlu\u011funda bir oligon\u00fckleotit ve 50 mM Na\u207a i\u00e7eren tampon \u00e7\u00f6zelti. Dizilimde 5 A, 4 T, 3 G ve 3 C baz\u0131 bulundu\u011fundan \\(G + C = 6\\), \\(A + T = 9\\) ve \\(n = 15\\) olur.<\/p>\n<p>\\[ [\\mathrm{Na^+}] = 50\\ \\mathrm{mM} = 0.05\\ \\mathrm{mol\/L}, \\quad \\log_{10}(0.05) = -1.301 \\]<br \/>\n\\[ T_m = 100.5 + 41 \\cdot \\frac{6}{15} - \\frac{820}{15} + 16.6 \\cdot (-1.301) \\]<br \/>\n\\[ T_m = 100.5 + 16.4 - 54.67 - 21.60 = 40.6\\ \\mathrm{\u00b0C} \\]<\/p>\n<p><strong>Sonu\u00e7:<\/strong> erime s\u0131cakl\u0131\u011f\u0131 \\(T_m \\approx 40.6\\) \u00b0C, GC i\u00e7eri\u011fi ise %40,0. Basit Wallace kural\u0131 2 \u00d7 9 + 4 \u00d7 6 = 42 \u00b0C sonucunu verirdi, ancak bu kural 14 baz ve daha uzun dizilimler i\u00e7in \u00f6nerilmez.<\/p>\n<p>\n\r\n<\/section>\n<h2>Hesaplay\u0131c\u0131 Nas\u0131l Kullan\u0131l\u0131r?<\/h2>\n<ol>\n<li>Diziliminizi <strong>DNA Dizilimi (5\u2032\u21923\u2032)<\/strong> alan\u0131na yaz\u0131n veya yap\u0131\u015ft\u0131r\u0131n. Yaln\u0131zca A, C, G ve T harfleri kabul edilir; b\u00fcy\u00fck\/k\u00fc\u00e7\u00fck harf, bo\u015fluk ve sat\u0131r sonlar\u0131 yok say\u0131l\u0131r.<\/li>\n<li><strong>Tuz (Na\u207a) Konsantrasyonu<\/strong> alan\u0131na toplam monovalent tuz konsantrasyonunu mM cinsinden girin (varsay\u0131lan de\u011fer 50 mM). Bu de\u011fer yaln\u0131zca dizilim 14 baz veya daha uzunsa kullan\u0131l\u0131r.<\/li>\n<li>Sonu\u00e7 panelinden <strong>Erme S\u0131cakl\u0131\u011f\u0131n\u0131 (Tm)<\/strong> \u00b0C cinsinden ve <strong>GC \u0130\u00e7eri\u011fini<\/strong> y\u00fczde olarak okuyun.<\/li>\n<li>Uygulanan form\u00fcl\u00fc, baz bile\u015fimini ve yerine konan say\u0131lar\u0131 g\u00f6rmek i\u00e7in <strong>Hesaplama Ayr\u0131nt\u0131lar\u0131<\/strong>n\u0131 kontrol edin; b\u00f6ylece sonucu elle de do\u011frulayabilirsiniz.<\/li>\n<\/ol>\n<h2>Erime S\u0131cakl\u0131\u011f\u0131 Nedir?<\/h2>\n<p>Erime s\u0131cakl\u0131\u011f\u0131, yani Tm, bir \u00e7\u00f6zeltideki DNA dublekslerinin yar\u0131s\u0131n\u0131n tek zincirlere ayr\u0131ld\u0131\u011f\u0131, yar\u0131s\u0131n\u0131n ise e\u015fle\u015fmi\u015f kald\u0131\u011f\u0131 s\u0131cakl\u0131kt\u0131r. Dubleks kararl\u0131l\u0131\u011f\u0131n\u0131n do\u011frudan bir \u00f6l\u00e7\u00fcs\u00fcd\u00fcr: Tm ne kadar y\u00fcksekse, iki zinciri ay\u0131rmak i\u00e7in o kadar \u00e7ok \u0131s\u0131 gerekir. Tm, iki tamamlay\u0131c\u0131 DNA zincirinin birbirini g\u00fcvenilir bi\u00e7imde bulup ba\u011flanmas\u0131 gereken her durumda \u00f6nem ta\u015f\u0131r \u2014 PCR primer tasar\u0131m\u0131, hibridizasyon probu ko\u015fullar\u0131n\u0131n se\u00e7imi ya da k\u0131sa oligolar i\u00e7in tavlama s\u0131cakl\u0131klar\u0131n\u0131n planlanmas\u0131 gibi.<\/p>\n<p>Dubleks kararl\u0131l\u0131\u011f\u0131 do\u011frudan baz \u00e7iftlerinden gelir. G\u2013C \u00e7ifti \u00fc\u00e7 hidrojen ba\u011f\u0131yla tutulurken A\u2013T \u00e7ifti yaln\u0131zca iki ba\u011f\u0131yla tutulur; bu y\u00fczden GC bak\u0131m\u0131ndan zengin dizilimler daha y\u00fcksek s\u0131cakl\u0131klarda erir. Wallace kural\u0131nda bu \u00e7ok a\u00e7\u0131k bi\u00e7imde ifade edilir: her A\u2013T \u00e7ifti Tm'ye yakla\u015f\u0131k 2 \u00b0C, her G\u2013C \u00e7ifti yakla\u015f\u0131k 4 \u00b0C ekler. Tm ile birlikte verilen GC i\u00e7eri\u011fi, dizilimin nas\u0131l olu\u015ftu\u011funu g\u00f6sterir \u2014 GC i\u00e7eri\u011fi ile Tm her zaman birlikte art\u0131p azald\u0131\u011f\u0131ndan, bu h\u0131zl\u0131 bir sa\u011flama y\u00f6ntemidir.<\/p>\n<p>Tuz da \u00f6nemli bir rol oynar. Sodyum iyonlar\u0131, fosfat omurgas\u0131ndaki negatif y\u00fckleri etkisiz k\u0131lar; bu nedenle daha y\u00fcksek Na\u207a konsantrasyonu dubleksi stabilize eder ve Tm'yi y\u00fckseltir. Tuz d\u00fczeltmeli form\u00fcl\u00fcn \\(16.6\\,\\log_{10}[\\mathrm{Na^+}]\\) terimini i\u00e7ermesinin nedeni budur: tuz konsantrasyonu her on kat art\u0131\u015f\u0131nda tahmin edilen Tm yakla\u015f\u0131k 16,6 \u00b0C y\u00fckselir. Oligolar\u0131 kar\u015f\u0131la\u015ft\u0131r\u0131rken daima ayn\u0131 tuz konsantrasyonunda kar\u015f\u0131la\u015ft\u0131r\u0131n ve tuz konsantrasyonunun mM cinsinden girilmesi gerekti\u011fini unutun \u2014 mol\/L'ye d\u00f6n\u00fc\u015f\u00fcm otomatik olarak yap\u0131l\u0131r.<\/p>\n<h2>Do\u011fruluk, Varsay\u0131mlar ve Pratik \u0130pu\u00e7lar\u0131<\/h2>\n<ul>\n<li><strong>Her iki form\u00fcl de kusursuz bir dubleks varsayar:<\/strong> dizilimin, uyu\u015fmayan baz, kabarc\u0131k (bulge) veya bo\u015fluk i\u00e7ermeyen, tamamen tamamlay\u0131c\u0131 bir zincirle hibridize olmas\u0131 gerekir. Tek bir uyu\u015fmazl\u0131k bile ger\u00e7ek Tm'yi birka\u00e7 derece d\u00fc\u015f\u00fcrebilir.<\/li>\n<li><strong>Wallace kural\u0131n\u0131n s\u0131n\u0131rlar\u0131:<\/strong> yakla\u015f\u0131k 4\u201313 baz uzunlu\u011funda ve 50 mM Na\u207a civar\u0131ndaki oligolar i\u00e7in tasarlanm\u0131\u015f kaba bir pratik kurald\u0131r. Tuz konsantrasyonunu ve dizilim uzunlu\u011funun etkilerini yok sayar; bu y\u00fczden yaln\u0131zca \u00e7ok k\u0131sa oligolar\u0131n h\u0131zl\u0131 tahmininde kullan\u0131n.<\/li>\n<li><strong>Tuz d\u00fczeltmeli form\u00fcl\u00fcn s\u0131n\u0131rlar\u0131:<\/strong> uzunlu\u011fu ve monovalent tuzu hesaba katar, ancak oligo konsantrasyonunu, u\u00e7 etkilerini, denat\u00fcranlar\u0131 (formamid, DMSO) ve PCR tamponlar\u0131nda dubleksleri belirgin bi\u00e7imde stabilize eden Mg\u00b2\u207a gibi divalent katyonlar\u0131 dikkate almaz.<\/li>\n<li><strong>Hassas \u00e7al\u0131\u015fmalar i\u00e7in en yak\u0131n kom\u015fu modelini kullan\u0131n:<\/strong> modern primer tasar\u0131m yaz\u0131l\u0131mlar\u0131, her din\u00fckleotit ad\u0131m\u0131n\u0131n y\u0131\u011f\u0131lma (stacking) enerjisini hesaba katan en yak\u0131n kom\u015fu (nearest-neighbour) termodinami\u011fini kullan\u0131r. Buradaki iki form\u00fcl birka\u00e7 derece hassasiyetle h\u0131zl\u0131 yakla\u015f\u0131mlard\u0131r \u2014 planlama ve \u00e7apraz kontrol i\u00e7in idealdir, nihai deney tasar\u0131m\u0131 i\u00e7in de\u011fil.<\/li>\n<li><strong>Sonucun yorumlanmas\u0131:<\/strong> pratik bir kural olarak hibridizasyon veya tavlama, Tm'nin yakla\u015f\u0131k 5 \u00b0C alt\u0131nda en iyi \u00e7al\u0131\u015f\u0131r. Hesaplanan Tm hedefinizden uzaksa, tavlama s\u0131cakl\u0131\u011f\u0131n\u0131 tek ba\u015f\u0131na de\u011fi\u015ftirmek yerine primer uzunlu\u011funu veya GC i\u00e7eri\u011fini ayarlay\u0131n.<\/li>\n<\/ul>\n<p>Ayn\u0131 deneylerde peptit veya proteinlerle de \u00e7al\u0131\u015f\u0131yorsan\u0131z, elektroforez ko\u015fullar\u0131n\u0131 planlamak i\u00e7in <a href=\"https:\/\/wpcalc.com\/tr\/kimya\/izoelektrik-nokta-hesaplama\/\">\u0130zoelektrik Nokta Hesaplay\u0131c\u0131m\u0131z<\/a>, hibridizasyon tamponunuzun \u0131s\u0131tma s\u0131ras\u0131nda pH'\u0131n\u0131 koruyup korumad\u0131\u011f\u0131n\u0131 kontrol etmek i\u00e7in de <a href=\"https:\/\/wpcalc.com\/tr\/kimya\/tampon-kapasitesi-hesaplayici\/\">Tampon Kapasitesi Hesaplay\u0131c\u0131s\u0131<\/a> i\u015finize yarayabilir.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Bir DNA oligon\u00fckleotidinin erime s\u0131cakl\u0131\u011f\u0131n\u0131 (Tm), dizilimi ve tuz konsantrasyonundan tahmin edin. Tek zinciri (A, C, G, T) ve Na\u207a konsantrasyonunu girin; Tm ve GC i\u00e7eri\u011fini an\u0131nda g\u00f6r\u00fcn.<\/p>\n","protected":false},"author":3168,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[3],"tags":[22],"class_list":["post-3963","post","type-post","status-publish","format-standard","hentry","category-kimya","tag-biyokimya"],"acf":[],"_links":{"self":[{"href":"https:\/\/wpcalc.com\/tr\/wp-json\/wp\/v2\/posts\/3963","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wpcalc.com\/tr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wpcalc.com\/tr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wpcalc.com\/tr\/wp-json\/wp\/v2\/users\/3168"}],"replies":[{"embeddable":true,"href":"https:\/\/wpcalc.com\/tr\/wp-json\/wp\/v2\/comments?post=3963"}],"version-history":[{"count":0,"href":"https:\/\/wpcalc.com\/tr\/wp-json\/wp\/v2\/posts\/3963\/revisions"}],"wp:attachment":[{"href":"https:\/\/wpcalc.com\/tr\/wp-json\/wp\/v2\/media?parent=3963"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wpcalc.com\/tr\/wp-json\/wp\/v2\/categories?post=3963"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wpcalc.com\/tr\/wp-json\/wp\/v2\/tags?post=3963"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}